Analyzing Mitochondrial Morphology Through Simulation Supervised Learning

نویسندگان

چکیده

The quantitative analysis of subcellular organelles such as mitochondria in cell fluorescence microscopy images is a demanding task because the inherent challenges segmentation these small and morphologically diverse structures. In this article, we demonstrate use machine learning-aided pipeline for quantification mitochondrial morphology fixed cells. deep learning-based tool trained on simulated eliminates requirement ground truth annotations supervised learning. We utility cardiomyoblasts with stable expression fluorescent markers employ specific culture conditions to induce changes morphology.

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ژورنال

عنوان ژورنال: Journal of Visualized Experiments

سال: 2023

ISSN: ['1940-087X']

DOI: https://doi.org/10.3791/64880-v